Research Technician II

Tissue resident memory T cells (Trm) perform tactile immune surveillance in frontline mucosal tissues and play key roles in protection against infectious diseases and cellular transformation.

The Joag lab seeks to understand how the interplay between CD8 memory T cells and neighboring cells in mucosal tissues fortifies and diversifies tissue immunity against infectious diseases in some contexts but may exacerbate tissue pathology in others.

Job Responsibilities
Laboratory management duties including ordering, maintaining lab safety and training records, and developing and maintaining a lab database
Mouse colony breeding and maintenance
Assistance in writing and updating lab protocols
Plan and perform moderately complex experiments, analyze and interpret data; discuss results with the PI on a regular basis
Excellent organizational skills with the ability to maintain detailed, updated, and accurate records
Meet with Principal Investigator on a regular basis to discuss lab management, plan ongoing/proposed work, and discuss experimental findings
Synthesize experiments and results into presentations; in conjunction with the PI, assist in writing and editing manuscripts and grant proposals
Experience in multiparameter flow cytometry, tissue harvesting and processing, in vivo infection models and mouse husbandry, and cell culture: primary immune cells and cell lines
Requirements
Bachelor's Degree in Immunology or related Life Science disciplines such as Cell and Molecular Biology or Pathobiology with a minimum 1-year experience in a research laboratory

About Us
Seattle Children's is a mission-driven organization that is life-changing to many, and touches the hearts of all. We deliver superior patient care, advance new discoveries and treatments through pediatric research, and serve as the pediatric and adolescent, academic medical center for Washington, Alaska, Montana and Idaho.